TGEV corona virus ORF4 encodes a membrane protein that is incorporated into virions
Identifieur interne : 000065 ( France/Analysis ); précédent : 000064; suivant : 000066TGEV corona virus ORF4 encodes a membrane protein that is incorporated into virions
Auteurs : Murielle Godet [France] ; Rene L'Haridon [France] ; Jean-Francois Vautherot [France] ; Hubert Laude [France]Source :
- Virology [ 0042-6822 ] ; 1992.
Descripteurs français
- KwdFr :
- Alignement de séquences, Baculoviridae (génétique), Cadres ouverts de lecture, Données de séquences moléculaires, Gènes viraux, Hybridomes (immunologie), Oligodésoxyribonucléotides (), Peptides (immunologie), Protéines membranaires (), Protéines membranaires (génétique), Protéines membranaires (immunologie), Protéines virales (), Protéines virales (génétique), Protéines virales (immunologie), Protéines virales structurales (génétique), Séquence consensus, Séquence nucléotidique, Vecteurs génétiques, Virion (), Virus de la gastroentérite transmissible (génétique).
- MESH :
- Pascal (Inist)
- Alignement de séquences, Anticorps monoclonal, Cadre lecture ouvert, Cadres ouverts de lecture, Données de séquences moléculaires, Déterminant antigénique, Expression génique, Gènes viraux, Oligodésoxyribonucléotides, Protéine membranaire, Protéines membranaires, Protéines virales, Séquence aminoacide, Séquence consensus, Séquence nucléotidique, Vecteurs génétiques, Virion, Virus gastroentérite transmissible porc.
English descriptors
- KwdEn :
- Aminoacid sequence, Antigenic determinant, Baculoviridae (genetics), Base Sequence, Consensus Sequence, Gene expression, Genes, Viral, Genetic Vectors, Hybridomas (immunology), Membrane Proteins (chemistry), Membrane Proteins (genetics), Membrane Proteins (immunology), Membrane protein, Molecular Sequence Data, Monoclonal antibody, Oligodeoxyribonucleotides (chemistry), Open Reading Frames, Open reading frame, Peptides (immunology), Porcine transmissible gastroenteritis virus, Sequence Alignment, Transmissible gastroenteritis virus (genetics), Viral Proteins (chemistry), Viral Proteins (genetics), Viral Proteins (immunology), Viral Structural Proteins (genetics), Virion (chemistry).
- MESH :
- chemical , chemistry : Membrane Proteins, Oligodeoxyribonucleotides, Viral Proteins.
- chemistry : Virion.
- genetics : Baculoviridae, Membrane Proteins, Transmissible gastroenteritis virus, Viral Proteins, Viral Structural Proteins.
- immunology : Hybridomas, Membrane Proteins, Peptides, Viral Proteins.
- Base Sequence, Consensus Sequence, Genes, Viral, Genetic Vectors, Molecular Sequence Data, Open Reading Frames, Sequence Alignment.
- Teeft :
- Academic press, Acnpv, Acorf4, Amino, Amino acid sequence, Amino acid sequences, Amino acids, Ascites fluids, Assay, Baculovirus, Bronchitis virus, Cell extracts, Cell monolayers, Clone, Common feature, Consensus sequence, Core sequence, Coronavirus, Coronavirus genome, Coronavirus genomes, Coronavirus members, Coronavirus study group, Coronaviruses, Encode nonstructural proteins, Epitope, Eukaryotic proteins, Gastroenteritis, Gastroenteritis virus, Genome, Godet, Human coronavirus, Hybridoma, Hydrophobic polypeptide, Immunofluorescence, Immunoprecipitated, Integral membrane protein, Laude, Mabs, Mammalian cells, Matrix protein genes, Membrane protein, Molar ratio, Monoclonal, Monoclonal antibodies, Mrna, Nonreducing conditions, Nucleotide sequence, Open reading frame, Orf4, Orf4 polypeptide, Orf4 product, Orf4 protein, Orf4 sequence, Orfs, Other coronaviruses, Peptide, Peptide scanning, Plasmid, Polyclonal antibodies, Polypeptide, Preliminary experiments, Reading frames, Recombinant, Recombinant baculovirus, Recombinant baculoviruses, Recombinant orf4 protein, Sample buffer, Sequence analysis, Simian virus, Spike glycoprotein, Structural polypeptide, Surface fluorescence, Tgev, Tgev orf4, Tgev particles, Tgev virions, Transmembrane orientation, Transmissible gastroenteritis virus, Unique regions, Viral, Virion, Virology, Virus, Wild type acnpv.
Abstract
Abstract: The coding potential of the open reading frame ORF4 (82 amino acids) of transmissible gastroenteritis virus (TGEV) has been confirmed by expression using a baculovirus vector. Five monoclonal antibodies (MAbs) raised against the 10K recombinant product immunoprecipitated a polypeptide of a similar size in TGEV-infected cells. Immunofluorescence assays performed both on insect and mammalian cells revealed that ORF4 was a membrane-associated protein, a finding consistent with the prediction of a membrane-spanning segment in ORF4 sequence. Two epitopes were localized within the last 21 C-terminal residues of the sequence through peptide scanning and analysis of the reactivity of a truncated ORF4 recombinant protein. Since the relevant MAbs were found to induce a cell surface fluorescence, these data suggest that ORF4 may be an integral membrane protein having a Cexo-Nendo orientation. Anti-ORF4 MAbs were also used to show that ORF4 polypeptide may be detected in TGEV virion preparations, with an estimated number of 20 molecules incorporated per particle. Comparison of amino acid sequence data provided strong evidence that other coronaviruses encode a polypeptide homologous to TGEV ORF4. Our results led us to propose that ORF4 represents a novel minor structural polypeptide, tentatively designated SM (small membrane protein).
Url:
DOI: 10.1016/0042-6822(92)90521-P
Affiliations:
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ISTEX:80743413DA1F1D29E7BBC0E2FD258B0C3BF66216Le document en format XML
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Virologie et lmmunologie Moléculaires, Institut National de la Recherche Agronomique, 78350 Jouy-en-Joses</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Jouy-en-Joses</settlement></placeName></affiliation></author><author><name sortKey="Vautherot, Jean Francois" sort="Vautherot, Jean Francois" uniqKey="Vautherot J" first="Jean-Francois" last="Vautherot">Jean-Francois Vautherot</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Unité de Virologie et lmmunologie Moléculaires, Institut National de la Recherche Agronomique, 78350 Jouy-en-Joses</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Jouy-en-Joses</settlement></placeName></affiliation></author><author><name sortKey="Laude, Hubert" sort="Laude, Hubert" uniqKey="Laude H" first="Hubert" last="Laude">Hubert Laude</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Unité de Virologie et lmmunologie Moléculaires, Institut National de la Recherche Agronomique, 78350 Jouy-en-Joses</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Jouy-en-Joses</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1992">1992</date><biblScope unit="volume">188</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="666">666</biblScope><biblScope unit="page" to="675">675</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aminoacid sequence</term><term>Antigenic determinant</term><term>Baculoviridae (genetics)</term><term>Base Sequence</term><term>Consensus Sequence</term><term>Gene expression</term><term>Genes, Viral</term><term>Genetic Vectors</term><term>Hybridomas (immunology)</term><term>Membrane Proteins (chemistry)</term><term>Membrane Proteins (genetics)</term><term>Membrane Proteins (immunology)</term><term>Membrane protein</term><term>Molecular Sequence Data</term><term>Monoclonal antibody</term><term>Oligodeoxyribonucleotides (chemistry)</term><term>Open Reading Frames</term><term>Open reading frame</term><term>Peptides (immunology)</term><term>Porcine transmissible gastroenteritis virus</term><term>Sequence Alignment</term><term>Transmissible gastroenteritis virus (genetics)</term><term>Viral Proteins (chemistry)</term><term>Viral Proteins (genetics)</term><term>Viral Proteins (immunology)</term><term>Viral Structural Proteins (genetics)</term><term>Virion (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alignement de séquences</term><term>Baculoviridae (génétique)</term><term>Cadres ouverts de lecture</term><term>Données de séquences moléculaires</term><term>Gènes viraux</term><term>Hybridomes (immunologie)</term><term>Oligodésoxyribonucléotides ()</term><term>Peptides (immunologie)</term><term>Protéines membranaires ()</term><term>Protéines membranaires (génétique)</term><term>Protéines membranaires (immunologie)</term><term>Protéines virales ()</term><term>Protéines virales (génétique)</term><term>Protéines virales (immunologie)</term><term>Protéines virales structurales (génétique)</term><term>Séquence consensus</term><term>Séquence nucléotidique</term><term>Vecteurs génétiques</term><term>Virion ()</term><term>Virus de la gastroentérite transmissible (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Proteins</term><term>Oligodeoxyribonucleotides</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Virion</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Baculoviridae</term><term>Membrane Proteins</term><term>Transmissible gastroenteritis virus</term><term>Viral Proteins</term><term>Viral Structural Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Baculoviridae</term><term>Protéines membranaires</term><term>Protéines virales</term><term>Protéines virales structurales</term><term>Virus de la gastroentérite transmissible</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Hybridomes</term><term>Peptides</term><term>Protéines membranaires</term><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Hybridomas</term><term>Membrane Proteins</term><term>Peptides</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Consensus Sequence</term><term>Genes, Viral</term><term>Genetic Vectors</term><term>Molecular Sequence Data</term><term>Open Reading Frames</term><term>Sequence Alignment</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Alignement de séquences</term><term>Anticorps monoclonal</term><term>Cadre lecture ouvert</term><term>Cadres ouverts de lecture</term><term>Données de séquences moléculaires</term><term>Déterminant antigénique</term><term>Expression génique</term><term>Gènes viraux</term><term>Oligodésoxyribonucléotides</term><term>Protéine membranaire</term><term>Protéines membranaires</term><term>Protéines virales</term><term>Séquence aminoacide</term><term>Séquence consensus</term><term>Séquence nucléotidique</term><term>Vecteurs génétiques</term><term>Virion</term><term>Virus gastroentérite transmissible porc</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Academic press</term><term>Acnpv</term><term>Acorf4</term><term>Amino</term><term>Amino acid sequence</term><term>Amino acid sequences</term><term>Amino acids</term><term>Ascites fluids</term><term>Assay</term><term>Baculovirus</term><term>Bronchitis virus</term><term>Cell extracts</term><term>Cell monolayers</term><term>Clone</term><term>Common feature</term><term>Consensus sequence</term><term>Core sequence</term><term>Coronavirus</term><term>Coronavirus genome</term><term>Coronavirus genomes</term><term>Coronavirus members</term><term>Coronavirus study group</term><term>Coronaviruses</term><term>Encode nonstructural proteins</term><term>Epitope</term><term>Eukaryotic proteins</term><term>Gastroenteritis</term><term>Gastroenteritis virus</term><term>Genome</term><term>Godet</term><term>Human coronavirus</term><term>Hybridoma</term><term>Hydrophobic polypeptide</term><term>Immunofluorescence</term><term>Immunoprecipitated</term><term>Integral membrane protein</term><term>Laude</term><term>Mabs</term><term>Mammalian cells</term><term>Matrix protein genes</term><term>Membrane protein</term><term>Molar ratio</term><term>Monoclonal</term><term>Monoclonal antibodies</term><term>Mrna</term><term>Nonreducing conditions</term><term>Nucleotide sequence</term><term>Open reading frame</term><term>Orf4</term><term>Orf4 polypeptide</term><term>Orf4 product</term><term>Orf4 protein</term><term>Orf4 sequence</term><term>Orfs</term><term>Other coronaviruses</term><term>Peptide</term><term>Peptide scanning</term><term>Plasmid</term><term>Polyclonal antibodies</term><term>Polypeptide</term><term>Preliminary experiments</term><term>Reading frames</term><term>Recombinant</term><term>Recombinant baculovirus</term><term>Recombinant baculoviruses</term><term>Recombinant orf4 protein</term><term>Sample buffer</term><term>Sequence analysis</term><term>Simian virus</term><term>Spike glycoprotein</term><term>Structural polypeptide</term><term>Surface fluorescence</term><term>Tgev</term><term>Tgev orf4</term><term>Tgev particles</term><term>Tgev virions</term><term>Transmembrane orientation</term><term>Transmissible gastroenteritis virus</term><term>Unique regions</term><term>Viral</term><term>Virion</term><term>Virology</term><term>Virus</term><term>Wild type acnpv</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The coding potential of the open reading frame ORF4 (82 amino acids) of transmissible gastroenteritis virus (TGEV) has been confirmed by expression using a baculovirus vector. Five monoclonal antibodies (MAbs) raised against the 10K recombinant product immunoprecipitated a polypeptide of a similar size in TGEV-infected cells. Immunofluorescence assays performed both on insect and mammalian cells revealed that ORF4 was a membrane-associated protein, a finding consistent with the prediction of a membrane-spanning segment in ORF4 sequence. Two epitopes were localized within the last 21 C-terminal residues of the sequence through peptide scanning and analysis of the reactivity of a truncated ORF4 recombinant protein. Since the relevant MAbs were found to induce a cell surface fluorescence, these data suggest that ORF4 may be an integral membrane protein having a Cexo-Nendo orientation. Anti-ORF4 MAbs were also used to show that ORF4 polypeptide may be detected in TGEV virion preparations, with an estimated number of 20 molecules incorporated per particle. Comparison of amino acid sequence data provided strong evidence that other coronaviruses encode a polypeptide homologous to TGEV ORF4. Our results led us to propose that ORF4 represents a novel minor structural polypeptide, tentatively designated SM (small membrane protein).</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Île-de-France</li></region><settlement><li>Jouy-en-Joses</li></settlement></list><tree><country name="France"><region name="Île-de-France"><name sortKey="Godet, Murielle" sort="Godet, Murielle" uniqKey="Godet M" first="Murielle" last="Godet">Murielle Godet</name></region><name sortKey="L Haridon, Rene" sort="L Haridon, Rene" uniqKey="L Haridon R" first="Rene" last="L'Haridon">Rene L'Haridon</name><name sortKey="Laude, Hubert" sort="Laude, Hubert" uniqKey="Laude H" first="Hubert" last="Laude">Hubert Laude</name><name sortKey="Vautherot, Jean Francois" sort="Vautherot, Jean Francois" uniqKey="Vautherot J" first="Jean-Francois" last="Vautherot">Jean-Francois Vautherot</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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